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	<title>Comments for SECular Thoughts</title>
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	<link>http://secularthoughts.wordpress.com</link>
	<description>A blog (mostly) about my graduate work on the secretory pathway</description>
	<lastBuildDate>Sat, 12 Sep 2009 00:24:56 +0000</lastBuildDate>
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		<title>Comment on Why yeast?  Part II by Veronika Miskolci</title>
		<link>http://secularthoughts.wordpress.com/2008/10/24/why-yeast-part-ii/#comment-70</link>
		<dc:creator>Veronika Miskolci</dc:creator>
		<pubDate>Sat, 12 Sep 2009 00:24:56 +0000</pubDate>
		<guid isPermaLink="false">http://secularthoughts.wordpress.com/?p=79#comment-70</guid>
		<description>Hi,
I would appreciate your explanation if you described the diploid knockout in a little bit more detail.  I am a first year graduate student taking molecular genetics.  The professor rushed through this technique.  What is confusing me is how do you study the function of the essential gene if you still have one good copy of it?  Why would it be called a knockout if you still have a copy?  I understand you need a copy to keep the cell alive.  Would the one copy give you an inpaired function, and that&#039;s how you infer the function of the essential gene?  Thank you!!!</description>
		<content:encoded><![CDATA[<p>Hi,<br />
I would appreciate your explanation if you described the diploid knockout in a little bit more detail.  I am a first year graduate student taking molecular genetics.  The professor rushed through this technique.  What is confusing me is how do you study the function of the essential gene if you still have one good copy of it?  Why would it be called a knockout if you still have a copy?  I understand you need a copy to keep the cell alive.  Would the one copy give you an inpaired function, and that&#8217;s how you infer the function of the essential gene?  Thank you!!!</p>
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		<title>Comment on So, what does an expensive microscope look like? by filmon daniel</title>
		<link>http://secularthoughts.wordpress.com/2008/03/31/so-what-does-an-expensive-microscope-look-like/#comment-59</link>
		<dc:creator>filmon daniel</dc:creator>
		<pubDate>Sun, 08 Feb 2009 14:53:26 +0000</pubDate>
		<guid isPermaLink="false">http://secularthoughts.wordpress.com/?p=46#comment-59</guid>
		<description>I want it now</description>
		<content:encoded><![CDATA[<p>I want it now</p>
]]></content:encoded>
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		<title>Comment on So, what does an expensive microscope look like? by adhanom semere</title>
		<link>http://secularthoughts.wordpress.com/2008/03/31/so-what-does-an-expensive-microscope-look-like/#comment-58</link>
		<dc:creator>adhanom semere</dc:creator>
		<pubDate>Sun, 08 Feb 2009 14:51:52 +0000</pubDate>
		<guid isPermaLink="false">http://secularthoughts.wordpress.com/?p=46#comment-58</guid>
		<description>hey,
    
   I was blown away with this microscope.I had never seen a microscope as good as this one.If you don&#039;t mind can you write the name of the microscope so that my mother could buy it for me in my birthday. My  father can&#039;t buy it because he dooes not live with me and my mom. He left us when i was only one year and a half. I heard he now lives in France with my three half sisters and my half brother. I try to contact him but he just ignors me, he doesn&#039;t even call me. I have never seen him for over nine years.</description>
		<content:encoded><![CDATA[<p>hey,</p>
<p>   I was blown away with this microscope.I had never seen a microscope as good as this one.If you don&#8217;t mind can you write the name of the microscope so that my mother could buy it for me in my birthday. My  father can&#8217;t buy it because he dooes not live with me and my mom. He left us when i was only one year and a half. I heard he now lives in France with my three half sisters and my half brother. I try to contact him but he just ignors me, he doesn&#8217;t even call me. I have never seen him for over nine years.</p>
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		<title>Comment on So, what does an expensive microscope look like? by Matt</title>
		<link>http://secularthoughts.wordpress.com/2008/03/31/so-what-does-an-expensive-microscope-look-like/#comment-57</link>
		<dc:creator>Matt</dc:creator>
		<pubDate>Wed, 28 Jan 2009 03:52:24 +0000</pubDate>
		<guid isPermaLink="false">http://secularthoughts.wordpress.com/?p=46#comment-57</guid>
		<description>Hello.

That is a very interesting set-up you have.  I am interested in purchasing something similar for my hobby--mycology.  Could you write down the specs and brand names/models so I could purchase something similar?  Price isn&#039;t an extreme impediment but I wouldn&#039;t want to spend over several thousand dollars.

Cheers,
Matt</description>
		<content:encoded><![CDATA[<p>Hello.</p>
<p>That is a very interesting set-up you have.  I am interested in purchasing something similar for my hobby&#8211;mycology.  Could you write down the specs and brand names/models so I could purchase something similar?  Price isn&#8217;t an extreme impediment but I wouldn&#8217;t want to spend over several thousand dollars.</p>
<p>Cheers,<br />
Matt</p>
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		<title>Comment on About by Greg Dyas</title>
		<link>http://secularthoughts.wordpress.com/about/#comment-56</link>
		<dc:creator>Greg Dyas</dc:creator>
		<pubDate>Wed, 12 Nov 2008 08:01:19 +0000</pubDate>
		<guid isPermaLink="false">#comment-56</guid>
		<description>Hi Elisabeth,

Nice blog!  Searched for you on a lark, and here you are blogging like a big bloggy thing.  I don&#039;t know if you&#039;ll remember me from BU, but thought it would be worth a shot to contact you from your blog comments.

It&#039;s nice to see you doing well, in grad school and enjoying life.  My wife Shelby (who interestingly is an absolute knitting nut as well) &amp; I live here in Simi Valley, have two rugrats (girls, 6 &amp; 2), and I work as a scientist in Amgen&#039;s Hematology research division.

And IMO the best reason to work with yeast is that it makes the lab smell vaguely of bread.  Please do email, I&#039;d enjoy hearing from you.

--Greg Dyas
  gldyas@yahoo.com</description>
		<content:encoded><![CDATA[<p>Hi Elisabeth,</p>
<p>Nice blog!  Searched for you on a lark, and here you are blogging like a big bloggy thing.  I don&#8217;t know if you&#8217;ll remember me from BU, but thought it would be worth a shot to contact you from your blog comments.</p>
<p>It&#8217;s nice to see you doing well, in grad school and enjoying life.  My wife Shelby (who interestingly is an absolute knitting nut as well) &amp; I live here in Simi Valley, have two rugrats (girls, 6 &amp; 2), and I work as a scientist in Amgen&#8217;s Hematology research division.</p>
<p>And IMO the best reason to work with yeast is that it makes the lab smell vaguely of bread.  Please do email, I&#8217;d enjoy hearing from you.</p>
<p>&#8211;Greg Dyas<br />
  <a href="mailto:gldyas@yahoo.com">gldyas@yahoo.com</a></p>
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		<title>Comment on No common ground by Coturnix</title>
		<link>http://secularthoughts.wordpress.com/2008/09/17/no-common-ground/#comment-50</link>
		<dc:creator>Coturnix</dc:creator>
		<pubDate>Wed, 17 Sep 2008 22:35:18 +0000</pubDate>
		<guid isPermaLink="false">http://secularthoughts.wordpress.com/?p=68#comment-50</guid>
		<description>Get your talking points &lt;a href=&quot;http://www.talkorigins.org/origins/faqs-qa.html&quot; rel=&quot;nofollow&quot;&gt;here&lt;/a&gt;.</description>
		<content:encoded><![CDATA[<p>Get your talking points <a href="http://www.talkorigins.org/origins/faqs-qa.html" rel="nofollow">here</a>.</p>
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	<item>
		<title>Comment on A different kind of microscope by marie joy usi</title>
		<link>http://secularthoughts.wordpress.com/2008/04/20/a-different-kind-of-microscope/#comment-46</link>
		<dc:creator>marie joy usi</dc:creator>
		<pubDate>Wed, 09 Jul 2008 14:53:22 +0000</pubDate>
		<guid isPermaLink="false">http://secularthoughts.wordpress.com/?p=49#comment-46</guid>
		<description>???????????????????</description>
		<content:encoded><![CDATA[<p>???????????????????</p>
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	<item>
		<title>Comment on Blog blah by Microscopes</title>
		<link>http://secularthoughts.wordpress.com/2008/03/17/blog-blah/#comment-25</link>
		<dc:creator>Microscopes</dc:creator>
		<pubDate>Sat, 22 Mar 2008 02:10:53 +0000</pubDate>
		<guid isPermaLink="false">http://secularthoughts.wordpress.com/?p=37#comment-25</guid>
		<description>Wow, i like this, its informative. Keep up...</description>
		<content:encoded><![CDATA[<p>Wow, i like this, its informative. Keep up&#8230;</p>
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		<title>Comment on Lab update 2-15-08 by Lab update 3-1-08 &#171; SECular Thoughts</title>
		<link>http://secularthoughts.wordpress.com/2008/02/16/lab-update-2-15-08/#comment-21</link>
		<dc:creator>Lab update 3-1-08 &#171; SECular Thoughts</dc:creator>
		<pubDate>Sun, 02 Mar 2008 05:10:16 +0000</pubDate>
		<guid isPermaLink="false">http://secularthoughts.wordpress.com/?p=29#comment-21</guid>
		<description>[...] still a lot left for me to do, though. Unfortunately, I&#8217;m still having problems with the deconvolution software. Fortunately (?) I&#8217;m not the only one having problems. While this is definitely inconvenient, [...]</description>
		<content:encoded><![CDATA[<p>[...] still a lot left for me to do, though. Unfortunately, I&#8217;m still having problems with the deconvolution software. Fortunately (?) I&#8217;m not the only one having problems. While this is definitely inconvenient, [...]</p>
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		<title>Comment on Probably not the actual lyrics by Laelaps</title>
		<link>http://secularthoughts.wordpress.com/2008/02/21/probably-not-the-actual-lyrics/#comment-19</link>
		<dc:creator>Laelaps</dc:creator>
		<pubDate>Thu, 21 Feb 2008 20:45:46 +0000</pubDate>
		<guid isPermaLink="false">http://secularthoughts.wordpress.com/?p=30#comment-19</guid>
		<description>I always heard the lyric &quot;There&#039;s a bad moon on the rise&quot; in the CCR song correctly until someone told me that they thought it was &quot;There&#039;s a bathroom on the right.&quot; Now I can&#039;t stop hearing the &quot;alternate&quot; version...</description>
		<content:encoded><![CDATA[<p>I always heard the lyric &#8220;There&#8217;s a bad moon on the rise&#8221; in the CCR song correctly until someone told me that they thought it was &#8220;There&#8217;s a bathroom on the right.&#8221; Now I can&#8217;t stop hearing the &#8220;alternate&#8221; version&#8230;</p>
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